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-------- Mensagem Original --------
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<th valign="BASELINE" align="RIGHT" nowrap="nowrap">Assunto:
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<td>Integrating Biomarkers and Cell Physiology Workshop -
21st November</td>
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<th valign="BASELINE" align="RIGHT" nowrap="nowrap">Data: </th>
<td>Tue, 12 Aug 2014 11:35:20 +0100</td>
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<th valign="BASELINE" align="RIGHT" nowrap="nowrap">De: </th>
<td>Divulgação BIOCANT <a class="moz-txt-link-rfc2396E" href="mailto:divulgacao@biocant.pt"><divulgacao@biocant.pt></a></td>
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<th valign="BASELINE" align="RIGHT" nowrap="nowrap">Para: </th>
<td>'Divulgação Biocant' <a class="moz-txt-link-rfc2396E" href="mailto:divulgacao@biocant.pt"><divulgacao@biocant.pt></a></td>
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<p class="MsoNormal"> <b> </b> <img id="Imagem_x0020_6"
src="cid:part1.09010104.09030007@eq.uc.pt"
alt="banner.jpg" width="905" height="152"><o:p></o:p></p>
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<p class="MsoNormal"><b><span
style="font-size:11.0pt;font-family:"Arial","sans-serif";color:#13CB25"
lang="EN-US">Center for Neuroscience and Cell
Biology (CNC) and Biocant invites you to attend
Integrating Biomarkers and Cell Physiology
Workshop!<o:p></o:p></span></b></p>
<p class="MsoNormal"><b><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#00B8C3"
lang="EN-US"><o:p> </o:p></span></b></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">The optimal energy state of a cell is
dependent on two main components. First, it
requires a functional intracellular infrastructure
in the form of intermediary metabolic pathway
enzymes and mitochondria that couple ATP
generation to substrate oxidation. <o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">Second, this system needs to be
flexible in matching the availability of
substrates and oxygen to cellular energy demand
thereby allowing cellular function to be
maintained under different nutrient and redox
states. <o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">In disease states, compromised
intracellular infrastructure and/or abnormal
substrate or oxygen availability can lead to
deficient energy production and cell function. <o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">Describing and explaining these
changes at a molecular level will aid disease
diagnosis and intervention. To this end, cellular
metabolic function can be described by the
following complementary measurements.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">Measurement of metabolite levels
informs the intracellular redox status, substrate
selection and rate-limiting steps of specific
metabolic fluxes.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">Measurement of oxygen consumption and
extracellular acidification inform the capacity of
the glycolytic-mitochondrial axis for producing
energy.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">In this workshop, we will learn and
discuss about two new analytical approaches that
have great potential for generating these data
from tissue, cell and organelle preparations under
standardized high-throughput conditions.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">Cetics has developed a
Fourier-transform infra-red spectroscopy (FTIR)
system that enables a metabolite fingerprint to be
rapidly and noninvasively obtained from tissue,
cell or organelle media. Another important
contribution from Cetics regards a high-throughput
genotoxicity analyzer which can provide
information on DNA damage or repair rates. <o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">Seahorse has developed a system that
incorporates real-time measurements of oxygen
consumption and extracellular acidification in a
multi-well format that provides measurements of
glycolytic flux and mitochondrial respiration for
tissue slices, cells or isolated organelles. <o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#0D0D0D"
lang="EN-US">While each of these technologies per
se represents a major advance in metabolic
characterization of tissues, cells and organelle
function, taken together they bridge the critical
pillars of intracellular substrate selection and
energy generation infrastructure.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#7F7F7F"
lang="EN-US"><o:p> </o:p></span></p>
<p class="MsoNormal"><span
style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"
lang="EN-US"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span
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lang="EN-US"><o:p> </o:p></span></b></p>
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<p class="MsoNormal"><b><span
style="font-size:4.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><a
moz-do-not-send="true"
href="http://www1.biocant.pt/integrating_metabonomics_cell_physiology/"><span
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