[Divulgacao] Fwd: Integrating Biomarkers and Cell Physiology Workshop - 21st November

[Mafalda Fernandes]

-------- Mensagem Original --------
Assunto: 	Integrating Biomarkers and Cell Physiology Workshop - 21st 
November
Data: 	Mon, 3 Nov 2014 10:56:31 -0000
De: 	Divulgação BIOCANT <divulgacao at biocant.pt>
Para: 	'Divulgação BIOCANT' <divulgacao at biocant.pt>



*EARLY INSCRIPTION DEADLINE – 5^th November*

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*Center for Neuroscience and Cell Biology (CNC) and Biocant invites you 
to attend Integrating Biomarkers and Cell Physiology Workshop!*

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The optimal energy state of a cell is dependent on two main components. 
First, it requires a functional intracellular infrastructure in the form 
of intermediary metabolic pathway enzymes and mitochondria that couple 
ATP generation to substrate oxidation.

Second, this system needs to be flexible in matching the availability of 
substrates and oxygen to cellular energy demand thereby allowing 
cellular function to be maintained under different nutrient and redox 
states.

In disease states, compromised intracellular infrastructure and/or 
abnormal substrate or oxygen availability can lead to deficient energy 
production and cell function.

Describing and explaining these changes at a molecular level will aid 
disease diagnosis and intervention. To this end, cellular metabolic 
function can be described by the following complementary measurements.

Measurement of metabolite levels informs the intracellular redox status, 
substrate selection and rate-limiting steps of specific metabolic fluxes.

Measurement of oxygen consumption and extracellular acidification inform 
the capacity of the glycolytic-mitochondrial axis for producing energy.

In this workshop, we will learn and discuss about two new analytical 
approaches that have great potential for generating these data from 
tissue, cell and organelle preparations under standardized 
high-throughput conditions.

Cetics has developed a Fourier-transform infra-red spectroscopy (FTIR) 
system that enables a metabolite fingerprint to be rapidly and 
noninvasively obtained from tissue, cell or organelle media. Another 
important contribution from Cetics regards a high-throughput 
genotoxicity analyzer which can provide information on DNA damage or 
repair rates.

Seahorse has developed a system that incorporates real-time measurements 
of oxygen consumption and extracellular acidification in a multi-well 
format that provides measurements of glycolytic flux and mitochondrial 
respiration for tissue slices, cells or isolated organelles.

While each of these technologies per se represents a major advance in 
metabolic characterization of tissues, cells and organelle function, 
taken together they bridge the critical pillars of intracellular 
substrate selection and energy generation infrastructure.

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<http://www1.biocant.pt/integrating_metabonomics_cell_physiology/>***

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